The HGP generated a 'reference' sequence of the human genome - specifically, it sequenced one representative version of all parts of each human chromosome (totaling ~3 billion bases). The 1-base overhangs produced by BmrI may be hard to ligate.How much did it cost to generate the first human genome sequence as part of the Human Genome Project? Sticky ends from different BglI sites may not be compatible.Ī C T G G G ( N ) 4 N T G A C C C ( N ) 4 G C C N N N N N G G C C G G N N N N N C C G Sticky ends from different BstAPI sites may not be compatible.īsaHI is typically used at 37☌, but is even more active at 60☌.Įfficient cleavage requires at least two copies of the NarI recognition sequence.Įfficient cleavage requires at least two copies of the PluTI recognition sequence. G C A N N N N N T G C C G T N N N N N A C G Prolonged incubation with NdeI may lead to removal of additional nucleotides. Sticky ends from different PfoI sites may not be compatible.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |